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Complementary DNA (cDNA) Synthesis has various application in bioinformatics as well as in genetics. cDNA is double-stranded DNA synthesized from a single stranded RNA. cDNA is mostly used for cloning eukaryotic genes in prokaryotes. It is used for transferring the cDNA which is used to code the protein to the donor cell when a specific protein in a cell fails to express that protein. The first step for many protocols in molecular biology is cDNA synthesis, notably gene expression analysis using real-time quantitative PCR (qPCR).